Ligand anchored poly(propyleneimine) dendrimers for brain targeting: Comparative in vitro and in vivo assessment.

The present investigation was aimed at developing various ligands-anchored dendrimers and comparing their brain targeting potential at one platform. Sialic acid (S), glucosamine (G) and concanavalin A (C) anchored poly(propyleneimine) (PPI) dendritic nanoconjugates were developed and evaluated for delivery of anti-cancer drug, paclitaxel (PTX) to the brain. MTT assay on U373MG human astrocytoma cells indicated IC50 values of 0.40, 0.65, 0.95, 2.00 and 3.50µM for PTX loaded SPPI, GPPI, CPPI, PPI formulations, and free PTX, respectively. The invivo pharmacokinetics and biodistribution studies in rats showed significantly higher accumulation of PTX in brain as compared to free PTX. The order of targeting potential of various ligands under investigation was found as sialic acid>glucosamine>concanavalin A. Thus, it can be concluded that sialic acid, glucosamine and Con A can be used as potential ligands to append PPI dendrimers for enhanced delivery of anticancer drugs to the brain for higher therapeutic outcome.

Design, modeling, and performance evaluation of a novel dye cell for a high repetition rate dye laser.

In this paper, a new dye cell for transverse pumping was designed, modeled, and its performance in a narrow spectral width dispersive resonator, pumped by a high repetition rate copper vapor laser, was investigated. The scheme essentially involves the profiling of the cubical glass and stainless steel cylindrical surface such that convex-plano contour be present near the optical pumping region. The design is an amalgamation of straight and curved periphery to enhance the dye solution flow stabilities near the dye laser axis. A computational fluid dynamics analysis of the liquid flow through this dye cell has been carried out. The dye laser outputs such as optical average power, spectral width and wavelength stability, tuning range, pulse shape, through this new dye cell was evaluated. The dye laser average power about 30 mW was fairly steady over the observation period of more than an hour. The dye laser short-term (1 min) spectral width was within 0.824 ± 0.075 GHz, while, in a long-term, more than an hour, drifted by about 180 MHz. The dye laser wavelength in short-term fluctuates within ±0.0065 nm whereas in a long-term, more than an hour, drifted by about 0.0105 nm. The dye laser tuning range was 10 nm with a sub GHz spectral width operation. The pulse shape of the dye laser follows the pump laser pulse profile. Thus, the dye laser has demonstrated fairly long-term stability, without the use of either low expansion material or close loop control on the output.